Modified Nucleosides
3'-O-Methylcytidine
Quick view. Cytidine derivative methylated at the ribose 3'-oxygen, with free 2'- and 5'-hydroxyl groups. CAS 20594-00-7. Catalog target purity: By inquiry. Availability: Inquiry only — contact OROGO to confirm current availability.
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Product profile
3'-O-Methylcytidine is classified as modified nucleosides (Formula C10H15N3O5; molecular weight 257.24 g/mol).
3'-O-Methylcytidine can be evaluated in terminally blocked nucleotide, cap-chemistry and analytical-reference workflows where 3′-O-methylation changes extension or end-group reactivity.
For 3'-O-Methylcytidine, the 3′-O-methyl group blocks normal 3′-hydroxyl extension; confirm that this behavior matches the intended enzyme or synthesis workflow.
Names and search terms
Confirmed or normalized name variants
- Cytidine, 3'-O-methyl-
- 3'-OMe-Cytidine
Useful catalog search terms
- 20594-00-7
Physicochemical data
| Sugar modification | Ribose 3'-O-methyl |
|---|---|
| Hydroxyl state | 2' and 5' hydroxyl groups unprotected |
| Nucleobase | Cytosine |
| InChIKey | RZJCFLSPBDUNDH-ZOQUXTDFSA-N |
| InChI | InChI=1S/C10H15N3O5/c1-17-8-5(4-14)18-9(7(8)15)13-3-2-6(11)12-10(13)16/h2-3,5,7-9,14-15H,4H2,1H3,(H2,11,12,16)/t5-,7-,8-,9-/m1/s1 |
| SMILES | CO[C@@H]1[C@H](O[C@H]([C@@H]1O)N2C=CC(=NC2=O)N)CO |
Release documentation
The current specification defines the analytical method and acceptance criteria; the released lot result is reported on the CoA.
Additional HPLC, MS or NMR documentation can be agreed during technical review where available.
Product-specific evaluation notes
Potential advantages
- 3′-O-methylation provides a defined blocked ribose terminus and is analytically distinguishable from the corresponding 2′-O-methyl isomer.
Method limitations
- The 3′-O-methyl group blocks normal 3′-hydroxyl extension; confirm that this behavior matches the intended enzyme or synthesis workflow.
These points describe 3'-O-Methylcytidine or its named chemical feature. Broader product-family guidance is maintained on the category page.
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Product-family literature
These publications provide peer-reviewed context for the Modified Nucleosides product family and its named chemistry. They are background reading only, not evidence for the exact product, supplied form, lot specification, availability or performance.
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