DNA Phosphoramidites
Fluorescein-dT Phosphoramidite
Quick view. Fluorescein-dT Phosphoramidite is a dipivaloyl-protected fluorescein-linked deoxyuridine phosphoramidite, CAS 1194507-30-6, with formula C79H89N6O17P. CAS 1194507-30-6. Catalog target purity: By inquiry. Availability: Inquiry only — contact OROGO to confirm current availability.
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Product profile
Fluorescein-dT Phosphoramidite is classified as dna phosphoramidites (Formula C79H89N6O17P; molecular weight 1425.60).
A protected fluorescein-dT phosphoramidite building block for incorporating a fluorescein-modified deoxyuridine residue during oligonucleotide synthesis.
For Fluorescein-dT Phosphoramidite, dye-labeled nucleotides or amidites are bulkier than native monomers; incorporation efficiency, label density, photobleaching and light exposure should be controlled.
Names and search terms
Confirmed or normalized name variants
- 5'-Dimethoxytrityloxy-5-[N-((3',6'-dipivaloylfluoresceinyl)-aminohexyl)-3-acrylimido]-2'-deoxyuridine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]phosphoramidite
- Fluorescein-dT amidite
- PD4-006
Useful catalog search terms
- 1194507-30-6
Physicochemical data
| Nucleoside | 5-Substituted 2'-deoxyuridine |
|---|---|
| Reporter group | Dipivaloyl-protected fluorescein |
| Linker | Aminohexyl-linked nucleobase substituent |
| 5' protection | Dimethoxytrityl |
| 3' functionality | 2-Cyanoethyl N,N-diisopropyl phosphoramidite |
| Phosphorus stereochemistry | Unspecified |
| PubChem CID | 102602188 |
| InChIKey | CADOGPWQRZBAGJ-WTCNENMSSA-N |
| InChI | InChI=1S/C79H89N6O17P/c1-49(2)85(50(3)4)103(96-42-20-39-80)102-66-46-69(100-67(66)48-95-78(53-21-16-15-17-22-53,54-25-29-56(93-11)30-26-54)55-27-31-57(94-12)32-28-55)84-47-52(71(88)83-75(84)92)24-38-68(86)81-40-18-13-14-19-41-82-70(87)51-23-35-60-63(43-51)79(101-72(60)89)61-36-33-58(97-73(90)76(5,6)7)44-64(61)99-65-45-59(34-37-62(65)79)98-74(91)77(8,9)10/h15-17,21-38,43-45,47,49-50,66-67,69H,13-14,18-20,40-42,46,48H2,1-12H3,(H,81,86)(H,82,87)(H,83,88,92)/b38-24+/t66-,67+,69+,103?/m0/s1 |
| SMILES | CC(C)N(C(C)C)P(OCCC#N)O[C@H]1C[C@@H](O[C@@H]1COC(C2=CC=CC=C2)(C3=CC=C(C=C3)OC)C4=CC=C(C=C4)OC)N5C=C(C(=O)NC5=O)/C=C/C(=O)NCCCCCCNC(=O)C6=CC7=C(C=C6)C(=O)OC78C9=C(C=C(C=C9)OC(=O)C(C)(C)C)OC1=C8C=CC(=C1)OC(=O)C(C)(C)C |
Release documentation
The current specification defines the analytical method and acceptance criteria; the released lot result is reported on the CoA.
Additional HPLC, MS or NMR documentation can be agreed during technical review where available.
Product-specific evaluation notes
Potential advantages
- Fluorescent labels enable non-radioactive detection and can reduce downstream staining or secondary-label steps.
Method limitations
- Dye-labeled nucleotides or amidites are bulkier than native monomers; incorporation efficiency, label density, photobleaching and light exposure should be controlled.
These points describe Fluorescein-dT Phosphoramidite or its named chemical feature. Broader product-family guidance is maintained on the category page.
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Feature and workflow background
These publications discuss a broader chemistry feature or workflow identified from the catalog name or product family. They are background reading only, not evidence for the exact OROGO product, supplied form, lot specification, availability or performance.
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Technology platforms
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