3'-O-Me Nucleosides
3'-O-Methylpseudouridine
Quick view. A pseudouridine nucleoside bearing a methyl ether at the 3'-oxygen position. CAS 51293-40-4. Catalog target purity: By inquiry. Availability: Inquiry only — contact OROGO to confirm current availability.
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Product profile
3'-O-Methylpseudouridine is classified as 3'-o-methyl pseudouridine nucleoside (Formula C10H14N2O6; molecular weight 258.23 g/mol).
3'-O-Methylpseudouridine is relevant to modified RNA, mRNA optimization, epitranscriptomic standards or immune-recognition studies depending on the format supplied.
For 3'-O-Methylpseudouridine, modification benefits are context-dependent; transcript length, substitution level, enzyme system and analytical purity can change the observed outcome.
Names and search terms
Confirmed or normalized name variants
- 3'-OMe-pseudouridine
Useful catalog search terms
- 51293-40-4
- RDRALVQCVMHTMG-VGRMVHKJSA-N
Physicochemical data
| PubChem CID | 175677452 |
|---|---|
| IUPAC name | 5-[(2S,3S,4S,5R)-3-hydroxy-5-(hydroxymethyl)-4-methoxyoxolan-2-yl]-1H-pyrimidine-2,4-dione |
| InChIKey | RDRALVQCVMHTMG-VGRMVHKJSA-N |
| InChI | InChI=1S/C10H14N2O6/c1-17-8-5(3-13)18-7(6(8)14)4-2-11-10(16)12-9(4)15/h2,5-8,13-14H,3H2,1H3,(H2,11,12,15,16)/t5-,6+,7+,8-/m1/s1 |
| SMILES | CO[C@@H]1[C@H](O[C@H]([C@@H]1O)C2=CNC(=O)NC2=O)CO |
Release documentation
The current specification defines the analytical method and acceptance criteria; the released lot result is reported on the CoA.
Additional HPLC, MS or NMR documentation can be agreed during technical review where available.
Product-specific evaluation notes
Potential advantages
- Base modifications can tune RNA stability, translation behavior, duplex properties or analytical discrimination versus native bases.
- 3′-O-methylation provides a defined blocked ribose terminus and is analytically distinguishable from the corresponding 2′-O-methyl isomer.
Method limitations
- Modification benefits are context-dependent; transcript length, substitution level, enzyme system and analytical purity can change the observed outcome.
- The 3′-O-methyl group blocks normal 3′-hydroxyl extension; confirm that this behavior matches the intended enzyme or synthesis workflow.
These points describe 3'-O-Methylpseudouridine or its named chemical feature. Broader product-family guidance is maintained on the category page.
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Feature and workflow background
These publications discuss a broader chemistry feature or workflow identified from the catalog name or product family. They are background reading only, not evidence for the exact OROGO product, supplied form, lot specification, availability or performance.
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Technology platforms
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