2'-O-Me Phosphoramidites
5'-O-DMTr-N4-benzoyl-5-methyl-2'-O-methylcytidine-3'-CE phosphoramidite
Quick view. A protected 5-methyl-2'-O-methylcytidine phosphoramidite bearing N4-benzoyl and 5'-DMTr protection with a 3'-cyanoethyl N,N-diisopropylphosphoramidite group. CAS 166593-57-3. Catalog target purity: By inquiry. Availability: Inquiry only — contact OROGO to confirm current availability.
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Product profile
5'-O-DMTr-N4-benzoyl-5-methyl-2'-O-methylcytidine-3'-CE phosphoramidite is classified as 2'-o-me phosphoramidites (Formula C48H56N5O9P; molecular weight 877.96).
5'-O-DMTr-N4-benzoyl-5-methyl-2'-O-methylcytidine-3'-CE phosphoramidite — Identity-defined protected 2'-O-methyl-5-methylcytidine CE phosphoramidite for research workflows involving solid-phase oligonucleotide synthesis.
For 5'-O-DMTr-N4-benzoyl-5-methyl-2'-O-methylcytidine-3'-CE phosphoramidite, excessive or poorly positioned substitution can reduce desired enzyme interactions or activity in some assay formats.
Names and search terms
Confirmed or normalized name variants
- 2'-OMe-5-Me-C(Bz) phosphoramidite
- 5-Me-DMTr-2'-O-Me-C(Bz)-CE phosphoramidite
- 5'-DMTr-N4-Bz-2'-OMe-5-Me-C phosphoramidite
- DMT-2'-OMe-5-Me-dC(Bz)-CE phosphoramidite
Useful catalog search terms
- CAS 166593-57-3
Physicochemical data
| Nucleoside | 5-Methyl-2'-O-methylcytidine |
|---|---|
| Sugar modification | 2'-O-Methyl |
| 5' protection | DMTr |
| Base protection | N4-Benzoyl |
| 3'-Coupling group | 2-Cyanoethyl N,N-diisopropylphosphoramidite |
| PubChem CID | 11967352 |
| InChIKey | GIWYLFDPXKHBKT-AENBYTAOSA-N |
| InChI | InChI=1S/C48H56N5O9P/c1-32(2)53(33(3)4)63(60-29-15-28-49)62-42-41(61-46(43(42)58-8)52-30-34(5)44(51-47(52)55)50-45(54)35-16-11-9-12-17-35)31-59-48(36-18-13-10-14-19-36,37-20-24-39(56-6)25-21-37)38-22-26-40(57-7)27-23-38/h9-14,16-27,30,32-33,41-43,46H,15,29,31H2,1-8H3,(H,50,51,54,55)/t41-,42-,43-,46-,63?/m1/s1 |
| SMILES | CC1=CN(C(=O)N=C1NC(=O)C2=CC=CC=C2)[C@H]3[C@@H]([C@@H]([C@H](O3)COC(C4=CC=CC=C4)(C5=CC=C(C=C5)OC)C6=CC=C(C=C6)OC)OP(N(C(C)C)C(C)C)OCCC#N)OC |
| Canonical SMILES | CC1=CN(C(=O)N=C1NC(=O)C2=CC=CC=C2)[C@H]3[C@@H]([C@@H]([C@H](O3)COC(C4=CC=CC=C4)(C5=CC=C(C=C5)OC)C6=CC=C(C=C6)OC)OP(N(C(C)C)C(C)C)OCCC#N)OC |
Release documentation
The current specification defines the analytical method and acceptance criteria; the released lot result is reported on the CoA.
Additional HPLC, MS or NMR documentation can be agreed during technical review where available.
Product-specific evaluation notes
Potential advantages
- 2′-O-methyl groups are widely used to increase nuclease resistance and modulate hybridization behavior.
Method limitations
- Excessive or poorly positioned substitution can reduce desired enzyme interactions or activity in some assay formats.
These points describe 5'-O-DMTr-N4-benzoyl-5-methyl-2'-O-methylcytidine-3'-CE phosphoramidite or its named chemical feature. Broader product-family guidance is maintained on the category page.
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Product-family literature
These publications provide peer-reviewed context for the 2'-O-Me Phosphoramidites product family and its named chemistry. They are background reading only, not evidence for the exact product, supplied form, lot specification, availability or performance.
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Technology platforms
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